https://ogma.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:51986 Wed 28 Feb 2024 14:50:44 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:25358 Alliumcepa through chromosomal aberration and comet assay using root meristem cells. A. cepa root tips were exposed to Tridol-S 3% and Tridol-S 6% with six different concentrations (0%, 0.005%, 0.01%, 0.02%, 0.03%, 0.04% and 0.05%) for 24 h. Cytogenetic effect endpoints such as mitotic index, and chromosomal aberrations were observed. Chromosomal aberrations in the control (untreated onion root tips) was negligible with the mitotic index (MI) value of 79.6%, while the MI significantly decreased in all the test concentrations of firefighting compounds. Genotoxicity assessment through comet assay also revealed that both the products were genotoxic with a significant increase in per cent tail DNA and olive tail moment. Among the test compounds, Tridol-S 3% was more toxic than Tridol-S 6%. To our knowledge, this is the first study on the cyto- and genotoxic effects of class B firefighting foams to A. cepa root meristem cells. This study also suggests that the toxicological studies using A. cepa root meristem cells can be employed for evaluating the toxicological impacts of firefighting foams in the environment.]]> Sat 24 Mar 2018 07:24:42 AEDT ]]> https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:44045 Allium cepa root meristem cells. The A. cepa root tips were exposed to 6 different concentrations (1–100 mg L⁻¹) of PFOS for 48 h. Reduction in mitotic index and chromosomal aberrations was measured as genotoxic endpoints in meristematic root cells. Exposure to PFOS significantly affected cell division by reducing the miotic index at higher concentrations (>10 mg L⁻¹). The median effect concentration of PFOS to elicit cytotoxicity based on the mitotic index was 43.2 mg L⁻¹. Exposure to PFOS significantly increased chromosomal aberrations at concentrations >25 mg L⁻¹. The common aberrations were micronuclei, vagrant cells, and multipolar anaphase. The alkaline comet assay revealed a genotoxic potential of PFOS with increased tail DNA percentage at concentrations >25 mg L⁻¹. To our knowledge, this is the first study to report the cyto-genotoxic potential of PFOS in higher plants.]]> Fri 07 Oct 2022 14:28:51 AEDT ]]>